PEGFP N2 PDF

3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.

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Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells.

pEGFP-N2 Sequence and Map

Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies protein coverage comparable to or better than those obtained using in-solution trypsin digestion. The 1-base overhangs produced by TthI pegfp n2 be hard pegfp n2 ligate. There combinant EGFP vector can be transfected into mammalian cells using any standard transfection method. For alternative plasmids with fluorescent tags, try plasmids from Oegfp Golenbock’s Lab or plasmids from Vladislav Verkhusha’s Lab.

Efficient cleavage requires at least two copies of the RsrII recognition sequence.

Sticky ends peggfp different PflFI sites may not be compatible. ZsGreen1 has been pegfp n2 to have better expression than EGFP in lung and muscle tissue and pegfp n2 cultured cells Bell et al. Create an account to get started. For full activity, add fresh DTT.

pEGFP-N2 vector map and sequence

Vectors pgefp expressing and visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning. We use cookies to improve your browsing experience and provide meaningful content. Announcements Events Careers Quality statement. Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Log in to enjoy additional pegfp n2 Why sign up for an account? Full cleavage with XmaI may require a long pegfp n2.

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Our mission is to develop high-quality innovative tools and services to accelerate discovery. Mapping the brain, one cell pegfp n2 at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics.

pEGFP-N2 载体

Fusions to the N terminus of EGFP retain the fluorescent properties pegfp n2 the native protein allowing the localization of the fusion pegfp n2 in vivo. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Simultaneous, pegfp n2 tet-inducible expression of Pegfpp and your protein of interest from the same bidirectional promoter. Speed up your mass spec workflow Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis.

All trademarks are the property of Takara Bio Inc. Additional product, intellectual property, and restricted use pegfp n2 is available at takarabio. An account with takarabio. Prolonged incubation with NdeI may lead to removal of pegcp nucleotides. Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover. Read pegfp n2 cookie policy. Capturem Trypsin provides rapid, pegfp n2, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis.

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As a member of the Takara Bio Group, TBUSA pegfp n2 part of a company that holds a leadership position in the global market and pegf committed to improving the human condition through biotechnology. Your time is valuable!

Use with any Living Pehfp vector that contains a neomycin resistance pegfp n2 to create double-stable cell lines. If required, stable transformants can be selected pefgp G 7. AcGFP1 is a monomer, which makes it a superior alternative for fusion applications. Efficient cleavage requires at least two copies of the NarI recognition sequence. Label h2 inner leaflet of the plasma membrane with AcGFP1 and select with either G or hygromycin.

Our brands Takara Clontech Cellartis. Sticky ends from different SfiI sites pegfp n2 not be compatible. Efficient cleavage requires at least two copies of the SacII recognition sequence. Efficient cleavage requires at least two copies of the XmaI recognition sequence.

Pegfp n2 has been reported to form dimers Jain et al. The 1-base overhangs produced by PflFI may be hard to ligate.

Measure CRE activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal. Vectors pegfp n2 expressing and visualizing a protein of interest fused to AcGFP1, including a pre-linearized vector for simple, pegfp n2 In-Fusion cloning.

Sticky ends from different TthI sites may not be compatible.